TRAIL Protein (AA 114-281)
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- Target See all TRAIL (TNFSF10) Proteins
- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 114-281
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Sequence
- VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL
- Characteristics
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ED50 < 40 ng/mL, measured by the cell growth inhibitory assay using RPMI-8226 cells, corresponding to a specific activity of > 2.5 x 10^4 units/mg.
AA 114-281, expressed with an N-terminal Met - Purity
- > 95 % as analyzed by SDS-PAGE and HPLC.
- Endotoxin Level
- < 0.2 EU/μg, determined by LAL method.
- Top Product
- Discover our top product TNFSF10 Protein
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Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 114-281) protein
TNFSF10 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 118-281), (Trimer) protein (His-DYKDDDDK Tag)TNFSF10 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 1-281) protein (His tag)custom-made TNFSF10 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 1-291) protein (His tag)custom-made TNFSF10 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 1-281) protein (GST tag)TNFSF10 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 114-281) protein (His-DYKDDDDK-Strep II Tag)TNFSF10 Origin: Human Host: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 118-291) protein (His tag)TNFSF10 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 95-281) protein (His tag)TNFSF10 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) proteinTNFSF10 Origin: Human Host: Escherichia coli (E. coli) Recombinant 90,00% SDS
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstituted in ddH2O or PBS at 100 μg/mL.
- Buffer
- Lyophilized after extensive dialysis against PBS.
- Storage
- -80 °C
- Storage Comment
- Lyophilized recombinant human TRAIL/Apo2L (rhTRAIL) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhTRAIL remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
- Expiry Date
- 6 months
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- Target
- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
- Alternative Name
- TRAIL/Apo2L (TNFSF10 Products)
- Background
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TRAIL/Apo2L, also known as Tumor Necrosis Factor Super-Family 10 (TNFSF10), is a pleiotropic cytokine thatbelongs to the TNF superfamily. The full length TRAIL expressed in vivo is a Type II transmembrane protein, although the soluble form also exists and functions. TRAIL has four major receptors: two death receptors DR4 and DR5, two decoy receptors DcR1 and DcR2. TRAIL binds to the death receptors, recruits the FAS-associated death domain, activates caspases 8 and 10, and eventually leads to apoptosis. Because of its antitumor potential, TRAIL is actively studied as a therapeutic agent. On the other hand, abnormal expression of TRAIL in small arteries can induce the proliferation of smooth muscle cells, resulting in increasing vascular remodeling and pulmonary arterial hypertension. Recombinant human TRAIL/Apo2L (rhTRAIL) produced in E. coli is a single non-glycosylated polypeptide chain containing 169 amino acids. A fully biologically active molecule, rhTRAIL has a molecular mass of 19.6 kDa analyzed by reducing SDS-PAGE.
Synonyms: TNF-related apoptosis-inducing Ligand, TNFSF10, Apo2 Ligand, TL2 - Molecular Weight
- 19.6 kDa, observed by reducing SDS-PAGE.
- UniProt
- P50591
- Pathways
- Apoptosis, Positive Regulation of Endopeptidase Activity
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