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VEGF 165 (AA 27-190) (Active) Protein

Recombinant protein expressed in CHO Cells.
Catalog No. ABIN2018441

Quick Overview for VEGF 165 (AA 27-190) (Active) Protein (ABIN2018441)

Target

VEGF 165

Protein Type

Recombinant

Biological Activity

Active

Origin

  • 19
  • 2
  • 1
Rat

Source

  • 9
  • 7
  • 2
  • 1
  • 1
  • 1
  • 1
CHO Cells

Purity

> 95 % as analyzed by SDS-PAGE and HPLC.
  • Protein Characteristics

    AA 27-190

    Characteristics

    ED50 < 4 ng/mL, measured in a cell proliferation assay using HUVEC cells, corresponding to a specific activity of >2.5 x 10^5 units/mg

    Endotoxin Level

    < 0.2 EU/μg, determined by LAL method.
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  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstituted in ddH2O or PBS at 100 μg/mL.

    Buffer

    Lyophilized after extensive dialysis against PBS.

    Storage

    -80 °C

    Storage Comment

    Lyophilized recombinant Rat Vascular Endothelial Growth Factor 165 (VEGF165) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rrVEGF165 should be stable up to 1 week at 4 °C or up to 2 months at -20 °C.

    Expiry Date

    6 months
  • Target

    VEGF 165

    Alternative Name

    Vascular Endothelial Growth Factor 165 (VEGF165)

    Background

    Vascular Endothelial Growth Factor (VEGF) is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, Vascular Endothelial Growth Factor (VEGF) plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates Vascular Endothelial Growth Factor (VEGF) in the induction of tumor metastasis and intra-ocular neovascular syndromes. Vascular Endothelial Growth Factor (VEGF) signals through the three receptors, fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product.
    Synonyms: Vascular Endothelial Growth Factor, VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen

    Molecular Weight

    35-48 kDa, observed by non-reducing SDS-PAGE.
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