YY1 Protein (AA 221-321) (His tag)

Catalog No. ABIN2115463

Recombinant YY1 protein expressed in Escherichia coli (E. coli).

Quick Overview for YY1 Protein (AA 221-321) (His tag) (ABIN2115463)

Target: YY1 (YY1 Transcription Factor (YY1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 221-321

Purification tag / Conjugate: This YY1 protein is labelled with His tag.

Purpose: Recombinant Human Transcriptional Repressor Protein YY1 (C-6His)

Sequence: MVTMWSSDEK KDIDHETVVE EQIIGENSPP DYSEYMTGKK LPPGGIPGID LSDPKQLAEF ARMKPRKIKE DDAPRTIACP HKGCTKMFRD NSAMRKHLHT HGLEHHHHHH

Characteristics: Recombinant Human Yin and Yang 1 protein/Transcriptional repressor protein YY1 is produced by our E.coli expression system and the target gene encoding Val221-Gly321 is expressed with a 6His tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 um filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 weeks

Target Details

Target: YY1 (YY1 Transcription Factor (YY1))

Alternative Name: YY1

Background: Alternative Names: Transcriptional repressor protein YY1,Delta transcription factor,INO80 complex subunit S,NF-E1,Yin and yang 1,INO80STranscriptional repressor protein YY1(YY1)contains 4 C2H2-type zinc fingers and belongs to the YY transcription factor family. Multifunctional transcription factor exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. The effect on transcription regulation of the protein is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression.
Synonyms: Transcriptional repressor protein YY1,Delta transcription factor,INO80 complex subunit S,NF-E1,Yin and yang 1,INO80S

Molecular Weight: 12.6 kDa

UniProt: P25490