Dihydrofolate Reductase Protein (DHFR)
Quick Overview for Dihydrofolate Reductase Protein (DHFR) (ABIN2128904)
Target
See all Dihydrofolate Reductase (DHFR) ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Dihydrofolate Reductase (DHFR) (AA 1-187) protein (His tag)
DHFR Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Dihydrofolate Reductase (DHFR) (AA 1-187) protein (GST tag)DHFR Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Dihydrofolate Reductase (DHFR) (AA 1-187) protein (His tag)DHFR Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Dihydrofolate Reductase (DHFR) (AA 2-187) protein (His-SUMO Tag)DHFR Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Dihydrofolate Reductase (DHFR) protein (Myc-DYKDDDDK Tag)DHFR Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Comment
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Biological activity: Specific activity is 1.5 - 2.5 units/mL and was obtained by measuring the oxidation of NADPH in absorbance at 340 nm during reaction. One unit will convert 1.0 umole of 7,8 dihydrofolate and beta-NADPH to 5,6,7,8-tetrahydrofolate and beta-NADP per minute at pH 6.5 at 25 DEg C.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0.1M NaCl, 2 mM DTT, and 30 % glycerol.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C/-20 °C/-80 °C
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- Dihydrofolate Reductase (DHFR)
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Alternative Name
- DHFR
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Molecular Weight
- 23.6 kDa (207aa), confirmed by MALDI-TOF.
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Pathways
- Mitotic G1-G1/S Phases
Target
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