Dihydrofolate Reductase Protein (DHFR) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719443

Product Details

Target: Dihydrofolate Reductase (DHFR)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Dihydrofolate Reductase protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human DHFR / DHFRP1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Dihydrofolate Reductase (DHFR)

Alternative Name: Dhfr,dhfrp1 (DHFR Products)

Synonyms: DHFR Protein, F10_orf160 Protein, ECK0049 Protein, JW0047 Protein, tmrA Protein, cb595 Protein, zgc:86637 Protein, DDBDRAFT_0187132 Protein, DDBDRAFT_0230113 Protein, DDB_0187132 Protein, DDB_0230113 Protein, CG14887 Protein, Dmel\\CG14887 Protein, dhfr Protein, DHFRP1 Protein, DYR Protein, 8430436I03Rik Protein, AA607882 Protein, AI662710 Protein, AW555094 Protein, Dhfr1 Protein, confers resistance to sulfonamides Protein, dihydrofolate reductase Protein, dihydrofolate reductase DhfR Protein, Dihydrofolate reductase Protein, dhfr Protein, DHFR Protein, dhfR Protein, folA Protein, dfrA Protein, LOC707382 Protein, CNJ01940 Protein, DDB_G0286755 Protein, Dhfr Protein, Mesil_0096 Protein, Arch_0408 Protein, Fbal_3367 Protein, Ilyop_0482 Protein, Lbys_2007 Protein, Riean_0733 Protein, Mrub_1855 Protein, Poras_0766 Protein, Halhy_3552 Protein, Mesop_3614 Protein, LOC609048 Protein

Background: Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 21.3 kDa

NCBI Accession: NP_000782

Pathways: Mitotic G1-G1/S Phases