Dihydrofolate Reductase Protein (DHFR) (AA 2-187) (His-SUMO Tag)

Catalog No. ABIN5709401

This Recombinant Dihydrofolate Reductase protein is expressed in Escherichia coli (E. coli).

Quick Overview for Dihydrofolate Reductase Protein (DHFR) (AA 2-187) (His-SUMO Tag) (ABIN5709401)

Target: Dihydrofolate Reductase (DHFR)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 %

Product Details

Protein Characteristics: AA 2-187

Purification tag / Conjugate: This Dihydrofolate Reductase protein is labelled with His-SUMO Tag.

Sequence: VGSLNCIVAV SQNMGIGKNG DLPWPPLRNE FRYFQRMTTT SSVEGKQNLV IMGKKTWFSI PEKNRPLKGR INLVLSRELK EPPQGAHFLS RSLDDALKLT EQPELANKVD MVWIVGGSSV YKEAMNHPGH LKLFVTRIMQ DFESDTFFPE IDLEKYKLLP EYPGVLSDVQ EEKGIKYKFE VYEKND

Purification: SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: Dihydrofolate Reductase (DHFR)

Alternative Name: DYR

Background: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.

Molecular Weight: 37.3 kDa

UniProt: P00374

Pathways: Mitotic G1-G1/S Phases