IL6RA Protein

Catalog No. ABIN2130109

This Recombinant IL6RA protein is produced in HEK-293 Cells.

Quick Overview for IL6RA Protein (ABIN2130109)

Target: IL6RA (Interleukin 6 Receptor, alpha (IL6RA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 98 % pure

Product Details

Sequence: LAPRRCPAQE VARGVLTSLP GDSVTLTCPG VEPEDNATVH WVLRKPAAGS HPSRWAGMGR RLLLRSVQLH DSGNYSCYRA GRPAGTVHLL VDVPPEEPQL SCFRKSPLSN VVCEWGPRST PSLTTKAVLL VRKFQNSPAE DFQEPCQYSQ ESQKFSCQLA VPEGDSSFYI VSMCVASSVG SKFSKTQTFQ GCGILQPDPP ANITVTAVAR NPRWLSVTWQ DPHSWNSSFY RLRFELRYRA ERSKTFTTWM VKDLQHHCVI HDAWSGLRHV VQLRAQEEFG QGEWSEWSPE AMGTPWTESR SPPAENEVST PMQALTTNKD DDNILFRDSA NATSLPVQD

Characteristics: Purified recombinant Human IL6 R alpha protein
Expression System: HEK cells
Bioactivity: The ED50 was determined by its ability to intensify the IL-6 induced growth inhibition of Mouse M1 cells is ? 5.0 ng/mL, in the presence of 20 ng/mL of rhIL-6.

Endotoxin Level: < 0.1 ng per μg (1 EU/μg).

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Comment:

Biological activity: The ED50 was determined by its ability to intensify the IL-6 induced growth inhibition of Mouse M1 cells is approx. 5.0 ng/mL, in the presence of 20 ng/mL of rhIL-6.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 1 PBS to a concentration of 0.1-1.0 mg/mL. When dissolved, dilute further into aqueous buffered solutions.

Buffer: Lyophilized from 1x PBS.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Target Details

Target: IL6RA (Interleukin 6 Receptor, alpha (IL6RA))

Alternative Name: IL6R alpha

Background: IL6 mediates its biological effects through the type I IL6 receptor system that consists of two chains, IL6Ra and gp130. The IL6Ra chain is the binding component specific to IL6, while the gp130 only transmits signals of IL6 when bound to IL6Ra. The gp130 also can transmit signals from LIF, OSM, CNTF, IL11 and CT-1 in conjunction with other receptor subunits. The low-affinity binding site for IL6 is composed of IL6Ra alone. IL6Ra is expressed in a wide range of cells including T cells, fibroblasts and macrophages. Soluble IL6Ra which consists of only the extracellular domain of the IL6Ra chain, acts as an agonist of IL6 activity at low concentrations.
Alternative Names: B cell stimulatory factor-2 protein, soluble IL-6 receptor alpha protein, IL-6, IL6, Interleukin 6R alpha protein, CD126 protein, IL-6R alpha protein, IL-6 protein, IL 6 protein, IL 6

Molecular Weight: 37.6 kDa

Pathways: JAK-STAT Signaling, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints