Cathepsin B Protein (CTSB) (AA 18-339) (His tag)
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- Target See all Cathepsin B (CTSB) Proteins
- Cathepsin B (CTSB)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 18-339
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Cathepsin B protein is labelled with His tag.
- Sequence
- AA 18-339
- Characteristics
- rh CTSB is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 36.7 kDa (pro-form) and 29 kDa (mature-form). The predicted N-terminus is Arg18 (pro-form) or Phe74 (mature-form). Due to glycosylation, The reducing (R) protein migrates as 43 kDa (pro-form) band in SDS-PAGE and there may be a 34 kDa (mature-form) band.
- Purity
- >95 % as determined by SDS-PAGE.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product CTSB Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 50 mM Tris, 150 mM NaCl, pH 8.0
- Handling Advice
- Please avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
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Characterization of Novel Piperidine-Based Inhibitor of Cathepsin B-Dependent Bacterial Toxins and Viruses." in: ACS infectious diseases, Vol. 4, Issue 8, pp. 1235-1245, (2018) (PubMed).
: "Identification of agents effective against multiple toxins and viruses by host-oriented cell targeting." in: Scientific reports, Vol. 5, pp. 13476, (2015) (PubMed).
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Characterization of Novel Piperidine-Based Inhibitor of Cathepsin B-Dependent Bacterial Toxins and Viruses." in: ACS infectious diseases, Vol. 4, Issue 8, pp. 1235-1245, (2018) (PubMed).
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- Target
- Cathepsin B (CTSB)
- Alternative Name
- Cathepsin B (CTSB Products)
- Background
- Cathepsin B (CTSB) is also known as APP secretase (APPS) and CPSB, is an enzymatic protein belonging to the peptidase C1 family. Cathepsin B / CTSB is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme. The catalytic activity of Cathepsin B / APPS contains: Hydrolysis of proteins with broad specificity for peptide bonds, Preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L), In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides. As a thiol protease, cathepsin B / CPSB is believed to participate in intracellular degradation and turnover of proteins and has also been implicated in tumor invasion and metastasis. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors.
- Molecular Weight
- 36.7 kDa & 29.5 kDa
- NCBI Accession
- NP_001899
- Pathways
- Activation of Innate immune Response, Toll-Like Receptors Cascades
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