FZD2 Protein (AA 24-156) (Fc Tag)

Catalog No. ABIN2181124

This Recombinant FZD2 protein is produced in HEK-293 Cells. There are 2 publications available.

Quick Overview for FZD2 Protein (AA 24-156) (Fc Tag) (ABIN2181124)

Target: FZD2 (Frizzled Family Receptor 2 (FZD2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: >95 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 24-156

Purification tag / Conjugate: This FZD2 protein is labelled with Fc Tag.

Sequence: AA 24-156

Characteristics: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 41.7 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Tris with Glycine, Arginine and NaCl,  pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

References

Henkel, Tatge, Schöttelndreier, Tao, Dong, Gerhard: "Receptor Binding Domains of TcdB from Clostridioides difficile for Chondroitin Sulfate Proteoglycan-4 and Frizzled Proteins Are Functionally Independent and Additive." in: Toxins, Vol. 12, Issue 12, (2020) (PubMed).

Tao, Zhang, Meraner, Tovaglieri, Wu, Gerhard, Zhang, Stallcup, Miao, He, Hurdle, Breault, Brass, Dong: "Frizzled proteins are colonic epithelial receptors for C. difficile toxin B." in: Nature, Vol. 538, Issue 7625, pp. 350-355, (2017) (PubMed).

Target Details

Target: FZD2 (Frizzled Family Receptor 2 (FZD2))

Alternative Name: Frizzled-2

Background: Frizzled-2 (FZD2) is also known as FzE2, which belongs to the G-protein coupled receptor Fz/Smo family. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. FZD2 contains one FZ (frizzled) domain. FZD2 may be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. The Lys-Thr-X-X-X-Trp motif of FZD2 interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.

Molecular Weight: 41.7 kDa

Pathways: WNT Signaling