MME Protein (AA 52-750)

Catalog No. ABIN2181505

Recombinant MME protein expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for MME Protein (AA 52-750) (ABIN2181505)

Target: MME (Membrane Metallo-Endopeptidase (MME))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: >95 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 52-750

Sequence: AA 52-750

Characteristics: rhCD10, with Gly-Pro at the N- terminus, has a calculated MW of 80 kDa. The predicted N-terminus is Tyr 52. The reducing (R) protein migrates as 90-100 kDa due to glycosylation.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 100 mM NaCl, pH 8.0

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).

References

Willard, Barrow, Zraika: "Improved glycaemia in high-fat-fed neprilysin-deficient mice is associated with reduced DPP-4 activity and increased active GLP-1 levels." in: Diabetologia, Vol. 60, Issue 4, pp. 701-708, (2017) (PubMed).

Target Details

Target: MME (Membrane Metallo-Endopeptidase (MME))

Alternative Name: Neprilysin

Target Type: Chemical

Background: Cluster of differentiation 10 (CD10) is also known as membrane metallo-endopeptidase, neutral endopeptidase (NEP), Neprilysin, and common acute lymphoblastic leukemia antigen (CALLA), is a 90-110- kDa type II transmembrane glycoprotein normally expressed by a variety of tissues, including epithelial cells of the prostate, kidney, intestine, endometrium, adrenal glands, and lung. This zinc-dependent metalloprotease enzyme cleaves peptide bonds on the amino side of hydrophobic residues and inactivates a variety of physiologically active secreted peptides. CD20 is thought to be the rate-limiting degrading enzyme of amyloid β peptide (Aβ) whose abnormal misfolding and aggregation in neural tissue has been implicated in the development of Alzheimer's disease (AD). CD10 is also identified as the common acute lymphoblastic leukemia antigen (CALLA) present on leukemic cells of pre-B phenotype, and thus serves as the most important biomarker in the diagnosis of human acute lymphocytic leukemia (ALL).

Molecular Weight: 80.0 kDa

NCBI Accession: NP_000893

Pathways: RTK Signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration