Sonic Hedgehog Protein (SHH) (AA 24-197) (His tag)

Catalog No. ABIN2181760

Product Details

Target: Sonic Hedgehog (SHH)

Protein Type: Recombinant

Protein Characteristics: AA 24-197

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Sonic Hedgehog protein is labelled with His tag.

Sequence: AA 24-197

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.4 kDa. The protein migrates as 22 kDa under reducing (R) condition (SDS-PAGE).

Purity: >95 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).

References

Kaushal, Popli, Sankhwar, Shukla, Dwivedi: "Sonic hedgehog protects endometrial hyperplasial cells against oxidative stress via suppressing mitochondrial fission protein dynamin-like GTPase (Drp1)." in: Free radical biology & medicine, Vol. 129, pp. 582-599, (2018) (PubMed).

Target Details

Target: Sonic Hedgehog (SHH)

Alternative Name: Sonic Hedgehog (SHH Products)

Background: Sonic hedgehog protein (SHH) is also known as HHG-1, which belongs to the hedgehog family. SHH is expressed in fetal intestine, liver, lung, and kidney and not expressed in adult tissues. Sonic hedgehog protein can be cleavage into sonic hedgehog protein N-product and sonic hedgehog protein C-product by autolysis. SHH binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development. The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.

Molecular Weight: 20.4 kDa

NCBI Accession: NP_000184

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development