TNF alpha Protein (AA 77-233) (Biotin)

Catalog No. ABIN2181833

This Recombinant TNF alpha protein is expressed in HEK-293 Cells and has been mentioned in 2+ publications.

Quick Overview for TNF alpha Protein (AA 77-233) (Biotin) (ABIN2181833)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: >95 % as determined by reduced SDS-PAGE.

Product Details

Protein Characteristics: AA 77-233

Purification tag / Conjugate: This TNF alpha protein is labelled with Biotin.

Brand: MABSol®,UltraLys

Sequence: AA 77-233

Specificity: The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Characteristics: The product does NOT contain any epitope tags. The protein has a calculated MW of 17.4 kDa. The protein migrates as 18 kDa on a SDS-PAGE gel under reducing (R) condition.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

A chemically labeled biotinylated protein with ultra sensitivity.
The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

References

Gao, Lu, Guo, Xin: "Dual-optofluidic waveguide in-line fiber biosensor for real-time label-free detection of interferon-gamma with temperature compensation." in: Optics express, Vol. 28, Issue 7, pp. 10491-10504, (2021) (PubMed).

El Amrani, van den Broek, Göbel, van Maarseveen et al.: "Quantification of active infliximab in human serum with liquid chromatography-tandem mass spectrometry using a tumor necrosis factor alpha -based pre-analytical sample purification and a stable ..." in: Journal of chromatography. A, Vol. 1454, pp. 42-8, (2016) (PubMed).

Target Details

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF-alpha

Background: Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.

Molecular Weight: 17.4 kDa

NCBI Accession: NP_000585

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome