VEGF Protein (AA 27-191) (His tag)

Catalog No. ABIN2181905

This Recombinant VEGF protein is produced in HEK-293 Cells. There is 1 publication available.

Quick Overview for VEGF Protein (AA 27-191) (His tag) (ABIN2181905)

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 27-191

Purification tag / Conjugate: This VEGF protein is labelled with His tag.

Sequence: AA 27-191

Characteristics: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 20 kDa (monomer). As a result of glycosylation, the protein migrates as 25 kDa (monomer) under reducing (R) condition, and 43-50 kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

References

Kerros, Tripathi, Zha, Mehrens, Sergeeva, Philips, Qiao, Peters, Katayama, Sukhumalchandra, Ruisaard, Perakis, St John, Lu, Mittendorf, Clise-Dwyer, Herrmann, Alatrash, Toniatti, Hanash, Ma, Molldrem: "Neuropilin-1 mediates neutrophil elastase uptake and cross-presentation in breast cancer cells." in: The Journal of biological chemistry, Vol. 292, Issue 24, pp. 10295-10305, (2017) (PubMed).

Target Details

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Alternative Name: VEGF

Background: VEGF165 is the most abundant splice variant of VEGF-A. VEGF165 is produced by a number of cells including endothelial cells, macrophages and T cells. VEGF165 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability. VEGF gene expression is induced by hypoxia, inflammatory cytokines and oncogenes. VEGF165 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix. VEGF165 binds to the receptor tyrosine kinases, VEGFR1 and VEGFR2. VEGF165 is the only splice variant that binds to co-receptors NRP-1 and NRP-2 that function to enhance VEGFR2 signaling. Binding of VEGF165 to VEGFR1 and VEGFR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK and paxillin. VEGF plays a key role in tumor angiogenesis in many cancers.

Molecular Weight: 21.1 kDa

NCBI Accession: NP_001165097