Taq RecA (Active) Protein

Catalog No. ABIN2452211

Product Details

Target: Taq RecA

Protein Type: Recombinant

Biological Activity: Active

Origin: Thermus aquaticus

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Characteristics: Single-strand dependent ATPase activity. The activity of single-stranded DNA-dependent ATPase was confirmed.

Purity: > 90 % of protein determined by SDS-PAGE (CBB staining)

Application Details

Application Notes: 1) Useful for studying homologous recombination
2) Increase the specificity and yield of multiplex PCR (of cDNA or genomic DNA) by promoting homologous annealing of primers to target DNA
3) Visualization of DNA with electoron microscopy due to nucleofilament formation.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 50 mM Tris-HCl ( pH 8.0), 200 mM NaCl, 1 mM EDTA, 50 % glycerol

Storage: -20 °C

References

Shigemori, Mikawa, Shibata, Oishi: "Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products." in: Nucleic acids research, Vol. 33, Issue 14, pp. e126, (2005) (PubMed).

Angov, Camerini-Otero: "The recA gene from the thermophile Thermus aquaticus YT-1: cloning, expression, and characterization." in: Journal of bacteriology, Vol. 176, Issue 5, pp. 1405-12, (1994) (PubMed).

Target Details

Target: Taq RecA

Background: Thermus aquaticus RecA protein is a thermostable enzyme which plays important roles in homologous recombination and DNA repair. This protein has activities of single-stranded DNA dependent ATPase, DNA annealing, and exchanging of strands between two recombining DNA double helices, similar to E.coli RecA protein, but the optimal temperature is between 65~75 °C. Taq RecA was expressed in E.coli in large quantities and the protein was highly purified. MW is 36.5kD.

UniProt: P48296