Taq RecA (Active) Protein
This Recombinant Taq RecA protein is produced in Escherichia coli (E. coli). There are 2 publications available.
1 image
(2 references)
Catalog No. ABIN2452211
$489.23
Plus shipping costs $50.00
100 μg
Shipping to:
United States
Delivery in 9 to 13 Business Days
Quick Overview for Taq RecA (Active) Protein (ABIN2452211)
Target
Taq RecA
Protein Type
Recombinant
Biological Activity
Active
Origin
Thermus aquaticus
Source
Escherichia coli (E. coli)
Application
Functional Studies (Func)
Purity
> 90 % of protein determined by SDS-PAGE (CBB staining)
-
-
Characteristics
- Single-strand dependent ATPase activity. The activity of single-stranded DNA-dependent ATPase was confirmed.
-
-
Want other Options for this Protein ?
!Discover Our Predefined Custom Proteins and Custom Protein Services!Your project requires further customization? Contact us and discover our custom protein solutions
-
-
Application Notes
-
1) Useful for studying homologous recombination
2) Increase the specificity and yield of multiplex PCR (of cDNA or genomic DNA) by promoting homologous annealing of primers to target DNA
3) Visualization of DNA with electoron microscopy due to nucleofilament formation. -
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Concentration
- 1 mg/mL
-
Buffer
- 50 mM Tris-HCl ( pH 8.0), 200 mM NaCl, 1 mM EDTA, 50 % glycerol
-
Storage
- -20 °C
-
-
-
: "Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products." in: Nucleic acids research, Vol. 33, Issue 14, pp. e126, (2005) (PubMed).
: "The recA gene from the thermophile Thermus aquaticus YT-1: cloning, expression, and characterization." in: Journal of bacteriology, Vol. 176, Issue 5, pp. 1405-12, (1994) (PubMed).
-
: "Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products." in: Nucleic acids research, Vol. 33, Issue 14, pp. e126, (2005) (PubMed).
-
- Taq RecA
-
Background
- Thermus aquaticus RecA protein is a thermostable enzyme which plays important roles in homologous recombination and DNA repair. This protein has activities of single-stranded DNA dependent ATPase, DNA annealing, and exchanging of strands between two recombining DNA double helices, similar to E.coli RecA protein, but the optimal temperature is between 65~75 °C. Taq RecA was expressed in E.coli in large quantities and the protein was highly purified. MW is 36.5kD.
-
UniProt
- P48296
Target
-
You are here:
