FGF acidic (Active) Protein
Origin: Rat
Host: Escherichia coli (E. coli)
Recombinant
< 95 % by SDS-PAGE gel and HPLC analyses.
Active
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- Target
- FGF acidic
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Rat
-
Source
- Escherichia coli (E. coli)
- Sequence
- MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
- Characteristics
- Biological activity was determined by a cell proliferation assay using balb/c 3T3 cells. The expected ED50 is < 0.1 ng/mL, in the presence of 10 μg/mL heparin, corresponding to a specific activity of > 1 x 107 units/mg.
- Purity
- < 95 % by SDS-PAGE gel and HPLC analyses.
- Endotoxin Level
- Endotoxin level is less than 0.1 ng per μg (1 EU/μg).
-
FGF acidic (AA 16-155) protein
Origin: Human Host: Escherichia coli (E. coli) Recombinant >98 % as determined by SDS-PAGE.
-
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Handling Advice
- As with any protein, exposing FGF-acidic recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
- Storage
- -20 °C
- Storage Comment
- The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted FGF-acidic stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.
- Expiry Date
- 24 months
-
- Target
- FGF acidic
- Alternative Name
- FGF-Acidic
- Background
- FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant rat FGF-acidic is a 15.9 kDa protein consisting of 141 amino acid residues.
- Gene ID
- 25317
- NCBI Accession
- NP_036978
- OMIM
- 6978839
- UniProt
- P61149
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