Kex-2 (Active) Protein

Catalog No. ABIN2468735

This Recombinant Kex-2 protein is produced in Hi-5 Cells.

Quick Overview for Kex-2 (Active) Protein (ABIN2468735)

Target: Kex-2

Protein Type: Recombinant

Biological Activity: Active

Origin: Saccharomyces cerevisiae

Source: Hi-5 Cells

Purity: < 95 % by SDS-PAGE gel and HPLC analyses.

Product Details

Sequence: APPMDSSLLP VKEAEDKLSI NDPLFERQWH LVNPSFPGSD INVLDLWYNN ITGAGVVAAI VDDGLDYENE DLKDNFCAEG SWDFNDNTNL PKPRLSDDYH GTRCAGEIAA KKGNNFCGVG VGYNAKISGI RILSGDITTE DEAASLIYGL DVNDIYSCSW GPADDGRHLQ GPSDLVKKAL VKGVTEGRDS KGAIYVFASG NGGTRGDNCN YDGYTNSIYS ITIGAIDHKD LHPPYSEGCS AVMAVTYSSG SGEYIHSSDI NGRCSNSHGG TSAAAPLAAG VYTLLLEANP NLTWRDVQYL SILSAVGLEK NADGDWRDSA MGKKYSHRYG FGKIDAHKLI EMSKTWENVN AQTWFYLPTL YVSQSTNSTE ETLESVITIS EKSLQDANFK RIEHVTVTVD IDTEIRGTTT VDLISPAGII SNLGVVRPRD VSSEGFKDWT FMSVAHWGEN GVGDWKIKVK TTENGHRIDF HSWRLKLFGE SIDSSKTETF VFGNDKEEVE PAATESTVSQ YSASSTSISI SATSTSSISI GVETSAIPQT TTASTDPDSD PNTP

Characteristics: Recombinant Kex-2 from High-5 insect cells contains the same specific activity and recognition sequence specificity as yeast derived KEX-2. 1 milligram of recombinant KEX-2 contains activity equivalent to at least 40 units of yeast derived KEX-2. Cleaves at the carboxyl side of K/R-R.

Endotoxin Level: Endotoxin level is less than 0.1 ng per μg (1 EU/μg).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Handling Advice: As with any protein, exposing Yeast Kex-2 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Storage: -20 °C

Storage Comment: The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted Yeast Kex-2 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.

Expiry Date: 24 months

Target Details

Target: Kex-2

Background: Proteases (also called Proteolytic Enzymes, Peptidases, or Proteinases) are enzymes that hydrolyze the amide bonds within proteins or peptides. Most proteases act in a specific manner, hydrolyzing bonds at or adjacent to specific residues or a specific sequence of residues contained within the substrate protein or peptide. Proteases play an important role in most diseases and biological processes including prenatal and postnatal development, reproduction, signal transduction, the immune response, various autoimmune and degenerative diseases, and cancer. They are also an important research tool, frequently used in the analysis and production of proteins. Kex-2 cleaves at the carboxyl end of the recognition sequences Arg-Arg/X and Lys-Arg/X. Recombinant Yeast Kex-2 is a 59.9 kDa protease consisting of 554 amino acid residues.

Gene ID: 855483

NCBI Accession: NP_014161

OMIM: 6324091

UniProt: P13134