Mycoplasma Arginine Deiminase (Active) Protein

Catalog No. ABIN2468872

Recombinant Mycoplasma Arginine Deiminase protein expressed in Escherichia coli (E. coli).

Quick Overview for Mycoplasma Arginine Deiminase (Active) Protein (ABIN2468872)

Target: Mycoplasma Arginine Deiminase

Protein Type: Recombinant

Biological Activity: Active

Origin: Mycoplasma crocodyli

Source: Escherichia coli (E. coli)

Purity: < 97 % by SDS-PAGE gel and HPLC analyses.

Product Details

Sequence: MSVFDSKFKG IHVYSEIGEL ESVLVHEPGR EIDYITPARL DELLFSAILE SHDARKEHKQ FVAELKANDI NVVELIDLVA ETYDLASQEA KDKLIEEFLE DSEPVLSEEH KVVVRNFLKA KKTSRELVEI MMAGITKTDL GIEADHELIV DPMPNLYFTR DPFASVGNGV TIHYMRYKVR QRETLFSRFV FSNHPKLINT PWYYDPSLKL SIEGGDVFIY NNDTLVVGVS ERTDLQTVTL LAKNIVANKE CEFKRIVAIN VPKWTNLMHL DTWLTMLDKD KFLYSPIAND VFKFWDYDLV NGGAEPQPVE NGLPLEGLLQ SIINKKPVLI PIAGEGASQM EIERETHFDG TNYLAIRPGV VIGYSRNEKT NAALEAAGIK VLPFHGNQLS LGMGNARCMS MPLSRKDVKW

Characteristics: Biological activity was measured by its ability to induce apoptosis in Jurkat cells using a concentration of 100-150 ng/mL.

Endotoxin Level: Endotoxin level is less than 0.1 ng per μg (1 EU/μg).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Handling Advice: As with any protein, exposing Mycoplasma Arginine Deiminase recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Storage: -20 °C

Storage Comment: The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted Mycoplasma Arginine Deiminase stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.

Expiry Date: 24 months

Target Details

Target: Mycoplasma Arginine Deiminase

Background: Arginine Deiminase (ADI) is a microbial enzyme from Mycoplasma produced in E.coli. It has high affinity to L-arginine and hydrolyzes L-arginine to citrulline and ammonia. Low concentrations of ADI have been shown to inhibit proliferation in certain cultured cells by arresting the cell cycle in G (1) and/or S phase. Higher concentrations of ADI lead to subsequent apoptosis. Recombinant Mycoplasma Arginine Deiminase is a 46.4 kDa protein consisting of 409 amino acids.

Gene ID: 8970640

NCBI Accession: YP_003560236

OMIM: 294155852

UniProt: D5E643