Oncostatin M Protein (OSM)

Catalog No. ABIN2468918

This Recombinant Oncostatin M protein is produced in Escherichia coli (E. coli).

Quick Overview for Oncostatin M Protein (OSM) (ABIN2468918)

Target: Oncostatin M (OSM)

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Escherichia coli (E. coli)

Purity: < 98 % by SDS-PAGE gel and HPLC analyses.

Product Details

Sequence: MKRGCSSSSP KLLSQLKSQA NITGNTASLL EPYILHQNLN TLTLRAACTE HPVAFPSEDM LRQLSKPDFL STVHATLGRV WHQLGAFRQQ FPKIQDFPEL ERARQNIQGI RNNVYCMARL LHPPLEIPEP TQADSGTSRP TTTAPGIFQI KIDSCRFLWG YHRFMGSVGR VFEEWGDGSR RSRRHSPLWA WLKGDHRIRP SRSSQSAMLR SLVPR

Characteristics: Assay 1: Biological activity was determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 is 3.0-5.0 μg/mL.
Assay 2: Biological activity was determined by its ability to inhibit Alkaline phosphatase activity of differentiated MC3T3 E1 cells.

Endotoxin Level: Endotoxin level is less than 0.1 ng per μg (1 EU/μg).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Handling Advice: As with any protein, exposing Oncostatin M recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Storage: -20 °C

Storage Comment: The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted Oncostatin M stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.

Expiry Date: 24 months

Target Details

Target: Oncostatin M (OSM)

Alternative Name: Oncostatin M

Background: Oncostatin M (OSM) is a growth and differentiation factor that participates in the regulation of neurogenesis, osteogenesis and hematopoiesis. Produced by activated T cells, monocytes and Kaposi s sarcoma cells, OSM can exert both stimulatory and inhibitory effects on cell proliferation. It stimulates the proliferation of fibroblasts, smooth muscle cells and Kaposi s sarcoma cells, but, inhibits the growth of some normal and tumor cell lines. It also promotes cytokine release (e.g. IL-6, GM-CSF and G-CSF) from endothelial cells, and enhances the expression of low-density lipoprotein receptor in hepatoma cells. OSM share several structural and functional characteristics with LIF, IL-6, and CNTF. Human OSM is active on murine cells. Recombinant rat Oncostatin M is a 24.4 kDa protein, containing 215 amino acid residues.

Gene ID: 289747

NCBI Accession: NP_001006962

OMIM: 55741439

UniProt: Q65Z15

Pathways: JAK-STAT Signaling, Negative Regulation of Hormone Secretion