Persephin Protein (PSPN)
PSPN
Origin: Mouse
Host: Escherichia coli (E. coli)
Recombinant
< 98 % by SDS-PAGE gel and HPLC analyses.
Active
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- Target See all Persephin (PSPN) Proteins
- Persephin (PSPN)
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Sequence
- ALAGSCRLWS LTLPVAELGL GYASEEKVIF RYCAGSCPQE ARTQHSLVLA RLRGRGRAHG RPCCQPTSYA DVTFLDDQHH WQQLPQLSAA ACGCGG
- Characteristics
- The ED50 was determined by its ability to stimulate proliferation of human thyroid carcinoma cells (TT cells) is < 0.1 ng/mL, corresponding to a specific activity of > 1 x 107 units/mg.
- Purity
- < 98 % by SDS-PAGE gel and HPLC analyses.
- Endotoxin Level
- Endotoxin level is less than 0.1 ng per μg (1 EU/μg).
- Top Product
- Discover our top product PSPN Protein
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Persephin (PSPN) (AA 61-156) protein (His tag)
PSPN Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Handling Advice
- As with any protein, exposing Persephin recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
- Storage
- -20 °C
- Storage Comment
- The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted Persephin is stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.
- Expiry Date
- 24 months
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- Target
- Persephin (PSPN)
- Alternative Name
- Persephin (PSPN Products)
- Synonyms
- PSPN Protein, PSP Protein, persephin Protein, PSPN Protein, Pspn Protein
- Background
- Persephin is a disulfide-linked homodimer neurotrophic factor structurally related to GDNF, Artemin, and Neurturin. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Persephin signals through a multicomponent receptor system, composed of RET and one of four GFRα (α1-α4) receptors. The GFRα4 was first identified in chicken and was later shown to be the preferential binding subunit for Persephin. Persephin promotes the survival of ventral midbrain dompaminergic neurons and motor neurons after sciatic nerve oxotomy, and like GNDF, promotes ureteric bud branching. However, in contrast to GDNF and Neurturin, Persephin does not support survival of peripheral neurons. Recombinant murine Persephin is a disulfide-linked homodimer, composed of two 10.3 kDa polypeptide chains (96 total amino acid residues). Each chain contains seven conserved cysteine residues, one of which (Cys 63) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration
- Gene ID
- 19197
- NCBI Accession
- NP_032980
- OMIM
- 247269886
- UniProt
- O70300
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