CD40 Ligand Protein (CD40LG) (Soluble)
Quick Overview for CD40 Ligand Protein (CD40LG) (Soluble) (ABIN2469037)
Target
See all CD40 Ligand (CD40LG) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- Soluble
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Sequence
- MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL
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Characteristics
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Assay 1: Determined by the stimulation of IL - 12 induction by human PBMCs.
Assay 2: Determined by the dose - dependent stimulation of IL - 8 production by human PBMC. The expected ED50 for this effect is 5 - 10 ng/mL. Note: Results may vary with PBMC donors. -
Endotoxin Level
- Endotoxin level is less than 0.1 ng per μg (1 EU/μg).
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CD40 Ligand (CD40LG) (AA 113-261), (Trimer) protein (Fc Tag)
CD40LG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD40 Ligand (CD40LG) (AA 113-261), (Trimer) protein (His-DYKDDDDK Tag,Biotin)CD40LG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD40 Ligand (CD40LG) (AA 113-261), (Trimer) protein (His-DYKDDDDK Tag)CD40LG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD40 Ligand (CD40LG) (AA 1-260) protein (His tag)custom-made CD40LG Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
CD40 Ligand (CD40LG) (AA 1-261) protein (His tag)custom-made CD40LG Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
CD40 Ligand (CD40LG) (AA 112-260), (Trimer) protein (Fc Tag)CD40LG Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD40 Ligand (CD40LG) (AA 116-261) protein (His-DYKDDDDK-Strep II Tag)CD40LG Origin: Human Host: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
CD40 Ligand (CD40LG) (AA 113-261) (Active) protein (Fc Tag)CD40LG Origin: Human, Rhesus Monkey Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD40 Ligand (CD40LG) (AA 116-261) (Active) protein (DYKDDDDK Tag,His tag)CD40LG Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD40 Ligand (CD40LG) proteinCD40LG Origin: Human Host: Human Cells Recombinant >90 % as determined by reducing SDS-PAGE.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Handling Advice
- As with any protein, exposing sCD40 Ligand recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
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Storage
- -20 °C
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Storage Comment
- The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted sCD40 Ligand is stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.
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Expiry Date
- 24 months
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- CD40 Ligand (CD40LG)
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Alternative Name
- CD40 Ligand
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Background
- CD40, a member of the TNF receptor family, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. The human CD40L gene codes for a 261 amino acid type II transmembrane protein, which contains a 22 amino acid cytoplasmic domain, a 24 amino acid transmembrane domain, and a 215 amino acid extracellular domain. The soluble form of CD40L is an 18 kDa protein comprising the entire TNF homologous region of CD40L and is generated in vivo by an intracellular proteolytic processing of the full length CD40L. Recombinant human soluble CD40 ligand is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.
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Gene ID
- 959
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NCBI Accession
- NP_000065
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OMIM
- 4557433
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UniProt
- P29965
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Pathways
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
Target
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