CD40 Ligand Protein (CD40LG) (Soluble)

Catalog No. ABIN2469083

This Recombinant CD40 Ligand protein is produced in Escherichia coli (E. coli).

Quick Overview for CD40 Ligand Protein (CD40LG) (Soluble) (ABIN2469083)

Target: CD40 Ligand (CD40LG)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: < 98 % by SDS-PAGE gel and HPLC analyses.

Product Details

Protein Characteristics: Soluble

Sequence: MQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLT VKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQ AGASVFVNVT EASQVIHRVG FSSFGLLKL

Characteristics: Biological activity was determined by its ability to induce TNF - alpha and MIP - 1 alpha production by murine splenocytes. The expected ED50 for this effect is < 0.1 μg/mL.

Endotoxin Level: Endotoxin level is less than 0.1 ng per μg (1 EU/μg).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Handling Advice: As with any protein, exposing Soluble CD40 Ligand recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Storage: -20 °C

Storage Comment: The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted Soluble CD40 Ligand is stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.

Expiry Date: 24 months

Target Details

Target: CD40 Ligand (CD40LG)

Alternative Name: CD40 Ligand

Background: CD40, a member of the TNF receptor family, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. The soluble form of CD40L is an 18 kDa protein comprising the entire TNF homologous region of CD40L and is generated in vivo by an intracellular proteolytic processing of the full length CD40L. Recombinant murine CD40L is a soluble 16.4 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.

Gene ID: 21939

NCBI Accession: NP_035741

OMIM: 70608202

UniProt: P27512

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints