CA2 Protein (His tag)

Catalog No. ABIN2566334

Recombinant CA2 protein expressed in HEK-293 Cells.

Quick Overview for CA2 Protein (His tag) (ABIN2566334)

Target: CA2 (Carbonic Anhydrase II (CA2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Western Blotting (WB)

Purity: >95 % as determined by SDS-PAGE.

Product Details

Purification tag / Conjugate: This CA2 protein is labelled with His tag.

Characteristics:

Measured by its esterase activity for digestion of 4-Nitrophenyl Acetate(4-NPA). The specific activity is > 150 pmoles/min/μg.

Application Details

Application Notes: This recombinant protein can be used for WB. For research use only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, pH 8.0, with 150 mM NaCl, 1 mM DTT.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -80 °C,-20 °C

Storage Comment: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

Target Details

Target: CA2 (Carbonic Anhydrase II (CA2))

Alternative Name: Carbonic Anhydrase 2

Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. They are, therefore, classified as metalloenzymes. There are at least five distinct CA families (α, β, γ, δ and ε). These families have no significant amino acid sequence similarity and in most cases are thought to be an example of convergent evolution. The α-CAs are found in humans. Carbonic anhydrase II (CA2) is also known as Carbonate dehydratase II, Carbonic anhydrase C, is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.

Molecular Weight: 30 kDa

Gene ID: 760

NCBI Accession: NP_000058

UniProt: P00918