CD14 Protein (CD14) (Fc Tag)

Catalog No. ABIN2566368

This Recombinant CD14 protein is produced in HEK-293 Cells.

Quick Overview for CD14 Protein (CD14) (Fc Tag) (ABIN2566368)

Target: CD14

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Western Blotting (WB)

Purity: >97 % as determined by SDS-PAGE.

Product Details

Purification tag / Conjugate: This CD14 protein is labelled with Fc Tag.

Characteristics:

Measured by its ability to enhance LPS-stimulated IL-8 secretion by THP-1 human acute monocytic leukemia cells. The ED50 for this effect is typically 0. 5-4 μg/mL.

Fusion tag: C-Fc Tag

Application Details

Application Notes: This recombinant protein can be used for WB. For research use only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 50 mM tris, 100 mM glycine, pH 7.5

Storage: -80 °C,-20 °C

Storage Comment: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

Target Details

Target: CD14

Alternative Name: CD14

Background: Cluster of differentiation 14 (CD14), is a cell surface glycoprotein, and is a is a component of the innate immune system. CD14 is a myelomonocytic differentiation antigen preferentially expressed on monocytes, macrophages, and activated granulocytes. CD14 exists in two forms. Either it is anchored into the membrane by a glycosylphosphatidylinositol tail (mCD14) or it appears in a soluble form (sCD14). Soluble CD14 either appears after shedding of mCD14 (48 kDa) or is directly secreted from intracellular vesicles (56 kDa). CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). CD14 has been proposed to be involved in various biological processes, including transportation of other lipids, cell-cell interaction during different immune responses, as well as recognition of apoptotic cells. Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns. CD14+ cells are monocytes that can differentiate into a host of different cells. CD14 has been shown to interact with Lipopolysaccharide-binding protein.

Molecular Weight: 62.4 kDa

Gene ID: 929

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades