DR4 Protein (Fc Tag)
DR4
Origin: Human
Host: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
WB, ELISA
Active
-
- Target See all DR4 products
- DR4 (Drought-Repressed 4 Protein (DR4))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This DR4 protein is labelled with Fc Tag.
- Application
- Western Blotting (WB), ELISA
- Characteristics
-
Measured by its binding ability in a functional ELISA. Immobilized human TNFSF10 at 10 μg/mL (100 μL/well ) can bind recombinant human TRAILR1 / TNFRSF10A Fc Chimera with a linear range of 0. 625 - 100 ng/mL.
Fusion tag: C-Fc Tag
- Purity
- >95 % as determined by SDS-PAGE.
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-
- Application Notes
- This recombinant protein can be used for WB, ELISA. For research use only.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 50 mM tris, 100 mM glycine, pH 7.5
- Storage
- -80 °C,-20 °C
- Storage Comment
- Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
-
- Target
- DR4 (Drought-Repressed 4 Protein (DR4))
- Alternative Name
- DR4 (DR4 Products)
- Synonyms
- APO2 Protein, CD261 Protein, DR4 Protein, TRAILR-1 Protein, TRAILR1 Protein, TNF receptor superfamily member 10a Protein, TNFRSF10A Protein
- Background
- Tumor necrosis factor receptor superfamily member 10A (TNFRSF10A) is also known as TNF-related apoptosis-inducing ligand receptor 1 (TRAIL-R1), Death receptor 4 (DR4), CD261 and APO2, which belongs to TNF superfamily. TRAILR1 / TNFRSF10A contains 1 death domain and 3 TNFR-Cys repeats. TNFRSF10A / DR4 is widely expressed and high levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K-562 erythroleukemia cells, MCF-7 breast carcinoma cells and activated T-cells. APO2 / TNFRSF10A is receptor for the cytotoxic ligand TNFSF10 / TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TRAILR-1 / DR4 / CD261 promotes the activation of NF-kappa-B.
- Molecular Weight
- 49 kDa
- Gene ID
- 8797
- NCBI Accession
- NP_003835
- UniProt
- O00220
- Pathways
- Apoptosis, Positive Regulation of Endopeptidase Activity
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