SPAM1 Protein (His tag)
Quick Overview for SPAM1 Protein (His tag) (ABIN2566867)
Target
See all SPAM1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This SPAM1 protein is labelled with His tag.
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Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1) (AA 346-445) protein (GST tag)
SPAM1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1) (AA 1-509) protein (GST tag)SPAM1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1) (AA 36-482) protein (Fc Tag)SPAM1 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1) (AA 36-482) (Active) protein (His tag)SPAM1 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)SPAM1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- This recombinant protein can be used for WB. For research use only.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- 50 mM Tris, 100 mM NaCl, pH 7.0-7.4
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Storage
- -80 °C,-20 °C
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Storage Comment
- Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
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- SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))
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Alternative Name
- PH20 Hyaluronidase
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Background
- Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N - acetyl - beta - D - glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
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Molecular Weight
- 52.1 kDa
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Gene ID
- 6677
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UniProt
- P38567
Target
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