MIA Protein
Quick Overview for MIA Protein (ABIN2648118)
Target
See all MIA ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Sequence
- MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQ
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Characteristics
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Purified recombinant Human MIA protein
Expression System: E.coli
Bioactivity: The ED50 was determined by the dose-dependant proliferation of the human A375 melanoma cell line. The expected ED50 for this effect is 4-6 µg/mL. -
Endotoxin Level
- < 0.1 ng per μg (1 EU/μg).
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Melanoma Inhibitory Activity (MIA) (Active) protein
MIA Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Melanoma Inhibitory Activity (MIA) (AA 1-131) protein (GST tag)MIA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Melanoma Inhibitory Activity (MIA) (Transcript Variant 1) (Active) proteinMIA Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining Func, AbP, STD, PI Active
Melanoma Inhibitory Activity (MIA) protein (Myc-DYKDDDDK Tag)MIA Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Comment
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Biological activity: The ED50 was determined by the dose-dependant proliferation of the human A375 melanoma cell line. The expected ED50 for this effect is 4-6 μg/mL.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in water to a concentration of 0.1-1.0 mg/mL.
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Buffer
- Lyophilized from 15 mM Na3PO4, pH 7.2 with 75 mM NaCl.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- RT/-20 °C
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- MIA (Melanoma Inhibitory Activity (MIA))
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Alternative Name
- MIA
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Background
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MIA is the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family, MIA, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. MIA is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of MIA may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis.
Alternative Names: Cartilage-derived retinoic acid-sensitive protein protein, CD-RAP protein, Melanoma Inhibitory Activity protein -
Molecular Weight
- 12.2 kDa
Target
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