MIA Protein (Transcript Variant 1)
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- Target See all MIA Proteins
- MIA (Melanoma Inhibitory Activity (MIA))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human MIA (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- ED50 was determined by the dose-dependant proliferation of the human A375 melanoma cell line. The expected ED50 for this effect is 4-6ug/mL.
- Top Product
- Discover our top product MIA Protein
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Melanoma Inhibitory Activity (MIA) (Active) protein
MIA Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Melanoma Inhibitory Activity (MIA) (AA 1-131) protein (GST tag)MIA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Melanoma Inhibitory Activity (MIA) protein (Myc-DYKDDDDK Tag)MIA Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- MIA (Melanoma Inhibitory Activity (MIA))
- Alternative Name
- Mia (MIA Products)
- Synonyms
- CD-RAP Protein, Mia1 Protein, Cdrap Protein, zgc:194157 Protein, mia Protein, CDRAP Protein, Fdp Protein, MIA Protein, MIAL Protein, melanoma inhibitory activity Protein, otoraplin Protein, cilia and flagella associated protein 100 Protein, MIA Protein, Mia Protein, mia Protein, Otor Protein, CFAP100 Protein
- Background
- Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas. [UniProtKB/Swiss-Prot Function]
- Molecular Weight
- 12.2 kDa
- NCBI Accession
- NP_006524
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