BMP4 Protein (AA 303-408)
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- Target See all BMP4 Proteins
- BMP4 (Bone Morphogenetic Protein 4 (BMP4))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 303-408
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- ELISA (Capture)
- Purity
- > 97 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Endotoxin Level
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Less than 0.1 ng per μg of protein.
- Top Product
- Discover our top product BMP4 Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Biological activity: BMP-4 induces alkaline phosphatase production on ATDC-5 cells. The expected ED50 for this effect is 5-10 ng/ml, corresponding to 1-2 x 105 unit/mg.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For maximum results, quick spin vial prior to opening. Reconstitute 5 mM HCl, pH 3.0 to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1 % BSA and store working aliquots at -20 °C to -80 °C.
- Buffer
- Lyophilized
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Unopened vial can be stored at -20°C or -70°C.
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- Target
- BMP4 (Bone Morphogenetic Protein 4 (BMP4))
- Alternative Name
- BMP-4 (BMP4 Products)
- Synonyms
- BMP2B Protein, BMP2B1 Protein, MCOPS6 Protein, OFC11 Protein, ZYME Protein, Bmp-4 Protein, Bmp2b Protein, Bmp2b-1 Protein, Bmp2b1 Protein, BOMPR4A Protein, bmp-4 Protein, zbmp-4 Protein, zgc:100779 Protein, BMP-4 Protein, XBMP-4 Protein, bmp2b Protein, bmp2b1 Protein, bmp4 Protein, ofc11 Protein, xbmp4 Protein, zyme Protein, BMP4 Protein, bone morphogenetic protein 4 Protein, bone morphogenetic protein 4 L homeolog Protein, bone morphogenetic protein 4 S homeolog Protein, BMP4 Protein, Bmp4 Protein, bmp4 Protein, bmp4.L Protein, bmp4.S Protein
- Background
- Bone morphogenetic proteins (BMPs) are multi-functional growth factors that belong to the transforming growth factor beta (TGFbeta) superfamily. BMPs play a key role in embryonic development, especially during heart, neural and cartilage development. Around 20 BMP family members have been identified and characterized. BMPs signal through serine/threonine kinase receptors, composed of type I and II subtypes. Four type I receptors have been identified: type IA and IB BMP receptors, type IA activin receptor, and activin receptor-like kinase I. Three type II receptors have also been recognized: type II BMP receptor and type II / IIB activin receptors. BMP-4 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, mouse, rat, and zebrafish. BMP-2 and BMP-4 are highly similar, they have 92 % amino acid sequence identity. BMP-4 is required for mesoderm formation and patterning in mice, and BMP-4 KO mice are embryonic-lethal. BMP-4 has an important role in postnatal tooth cytodifferentiation. Overexpression BMP-4 has been associated with fibrodysplasia ossificans progressiva (FOP). Overexpression of BMP-4 has also been associated with cancer, including melanoma, hepatocellular carcinoma, adenocarcinoma and colorectal cancer cell lines. This overexpression can promote migratory and invasive properties of cancer cells.
- Molecular Weight
- The 106 amino acid recombinant protein has a predicted molecular mass of approximately 12 kDa. The DTT-non reduced protein migrates at approximately 31-36 kDa on SDS-PAGE. The predicted N-terminal amino acid is Lys.
- Pathways
- Steroid Hormone Mediated Signaling Pathway, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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