IL-3 Protein (AA 27-169)

Catalog No. ABIN2666554

This Recombinant IL-3 protein is produced in Escherichia coli (E. coli).

Quick Overview for IL-3 Protein (AA 27-169) (ABIN2666554)

Target: IL-3 (Interleukin 3 (IL-3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 27-169

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: ED50 = 1 - 5 ng/ml, corresponding to a specific activity of 0.2 â€" 1.0 x 106 units/mg, as determined by the dose dependent stimulation of a M-NFS-60 cell proliferation assay.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20 °C to -70 °C. Stock solutions can also be prepared at 50 - 100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in PBS pH 7.2.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Target Details

Target: IL-3 (Interleukin 3 (IL-3))

Alternative Name: IL-3

Background: Interleukin 3, also known as IL-3, was initially described in the supernatant of cultures of viral-infected murine spleen cells. Mature rat IL-3 surprisingly shows low homology (54 % ) with its murine counterpart. IL-3 is the most potent growth factor for basophils followed by the granulocyte-macrophage colony-stimulating factor and IL-5. These cytokines also act on mature basophils through specific receptors, thereby mediating adhesion, migration, and release of the cells. IL-3 is highly expressed by mast cells. Rapid autocrine production of large amounts of the cytokine is responsible for mast cell IgE-induced survival in the absence of antigen. In addition, IL-3 and its receptors are expressed in neuronal progenitors and neurons. IL-3 has been associated to human brain volume variation by regulating proliferation and survival of neural progenitors. It has also been implicated in the pathogenesis of several chronic inflammatory diseases, which include asthma, atherosclerosis, and neurodegenerative disorders such as multiple sclerosis.

Molecular Weight: The 144 amino acid recombinant protein has a predicted molecular mass of approximately 16.3 kDa. The protein migrates at approximately 18 kDa in DTT-reducing conditions and 16 kDa in non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid

Pathways: JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy