TNFSF18 Protein (AA 74-199, N-Term)

Catalog No. ABIN2666573

This Recombinant TNFSF18 protein is expressed in HEK-293 Cells.

Quick Overview for TNFSF18 Protein (AA 74-199, N-Term) (ABIN2666573)

Target: TNFSF18 (Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Western Blotting (WB)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 74-199, N-Term

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 1.0 EU per μg of protein as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Human TNFSF18, when immobilized with anti-CD3 antibody, is able to inhibit IFN-γ secretion in human T cells in a dose-dependent manner with an ED50 of 0.5 - 4.0 μg/mL. Results may vary depending on human donors.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20 °C to -70 °C. Stock solutions can also be prepared at 50 - 100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in PBS, pH 7.2.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Target Details

Target: TNFSF18 (Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18))

Alternative Name: TNFSF18

Background: TNFSF18 is a type II transmembrane glycoprotein that belongs to the TNF superfamily. This protein is mainly expressed on antigen presenting cells (APC) including macrophages, B cells, dendritic cells (DCs), and endothelial cells. Its expression increases upon the stimulation of proinflammatory stimuli. The receptor of TNFSF18 is the glucocorticoid-induced TNFR-related protein (GITR), which is a member of the TNFR superfamily. Engagement of TNFSF18 and GITR leads to T cell activation, proliferation, and cytokine production. It also inhibits the suppressive function of T regulatory cells and induces macrophage activation. GITR-TNFSF18 interaction plays an essential role in the regulation of the immune response to self antigens and pathogens. In addition, this receptor-ligand interaction has been shown to modulate anti-tumor immunity.

Molecular Weight: The 147 amino acid recombinant protein has a predicted molecular mass of approximately 16.5 kDa. The protein migrates at about 25 kDa in DTT-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is His.