CXCL5 Protein (AA 45-118)

Catalog No. ABIN2666646

Recombinant CXCL5 protein expressed in Escherichia coli (E. coli).

Quick Overview for CXCL5 Protein (AA 45-118) (ABIN2666646)

Target: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Multiplex Assay (MA)

Purity: > 98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 45-118

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Bioactivity was measured by its property to chemoattract human neutrophils in a dose dependent manner.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Target Details

Target: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Alternative Name: CXCL5

Background: CXCL5 is an ELR+CXC chemokine characterized by a Glu-Leu-Arg (ELR) motif preceding the characteristic CXC sequence. CXCL5 chemoattracts and activates neutrophils during inflammation through the binding to CXCR2. CXCL5 occurs in different NH2- and COOH-truncated forms, and both NH2-terminal and COOH-terminal truncation result in an increase in neutrophil chemotactic potency. CXCL5(8-78) and CXCL5(9-78) induce a higher neutrophil influx in vivo compared with CXCL5(1-78). CXCL5 regulates the availability of binding sites for other ELR+ CXC chemokines released during inflammation, through its interaction with erythrocyte duffy antigen receptor (DARC). As a result, CXCL5 induces the increase of CXCL1 and CXCL2 in a model of pulmonary inflammation. Therefore, CXCL5 acts as a regulator of chemokine scavenging and pulmonary host defense to bacterial infection. Lung epithelial cells express CXCR2, consequently, CXCL5 may modulate neutrophil transepithelial and transendothelial migration. Mouse CXCL5 is the ortholog of human CXCL6.

Molecular Weight: The 74 amino acid recombinant protein has a predicted molecular mass of approximately 7.98 kDa. The DTT-reduced protein and non-reduced protein migrates at approximately 10 kDa by SDS-PAGE. The N-terminal amino acid is Val.

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity