IL-3 Protein (AA 27-166)

Catalog No. ABIN2666660

This Recombinant IL-3 protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL-3 Protein (AA 27-166) (ABIN2666660)

Target: IL-3 (Interleukin 3 (IL-3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Multiplex Assay (MA)

Purity: > 98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 27-166

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.01ng per μg cytokine as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: ED50 = 0.05 - 0.200 ng/ml, corresponding to a specific activity of 0.5-2.0 x 107 units/mg, as determined by the dose dependent stimulation of a M-NFS-60 cell proliferation assay.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22μm filtered protein solution is in 10 mM NaHPO4 pH 7.2, 0.15M NaCl

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Target Details

Target: IL-3 (Interleukin 3 (IL-3))

Alternative Name: IL-3

Background: IL-3 is the most potent growth factor for basophils followed by granulocyte-macrophage colony-stimulating factor and IL-5. These cytokines also act on mature basophils through specific receptors, thereby mediating adhesion, migration, and releasability. IL-3 is highly expressed by mast cells, and rapid and large amount of autocrine IL-3 production is responsible for mast cell survival by IgE in the absence of antigen. IL-3 has also been implicated in the pathogenesis of several chronic inflammatory diseases, including asthma, atherosclerosis, and neurodegenerative disorders, such as multiple sclerosis.

Molecular Weight: The 140 amino acid recombinant protein has a predicted molecular mass of 15673.7 Da. The DTT-reduced protein migrates at approximately 16 kDaa and the non-reduced protein migrates with slightly greater mobility by SDS-PAGE.

Pathways: JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy