IL-2 Protein (AA 21-153)

Catalog No. ABIN2666705

This Recombinant IL-2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL-2 Protein (AA 21-153) (ABIN2666705)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purity: Purity is > 98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 21-153

Sterility: 0.22 μm filtered

Endotoxin Level:

Endotoxin level is <0.1 EU/μg (<0.01ng/μg) protein as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: ED50 = 0.102 - 0.295 ng/ml, corresponding to a specific activity of 9.8 - 3.39 x 106 units/mg, as determined by the dose dependent stimulation of CTLL-2 cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in 5 mM NaH2PO4, 5 mM citric acid, 150 mM NaCl, pH 4.0.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Target Details

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL-2

Background: Interleukin 2 was discovered through its function as a T cell growth factor (TCGF). IL-2 binds to IL-2 receptor which is expressed in T and B cells, thymocytes, and NK cells. The IL-2 receptor comprises three distinct components: the α -chain, which is cytokine specific, and the β- and γc-subunits which are share with the IL-15 receptor. In addition, the γ c-subunit is a component of a series of other cytokine receptors, these being members of the γc cytokine receptor family (IL-4, IL-7, IL-9, and IL-21) (3). IL-2 signaling may play a major role in the differentiation of regulatory T cells (4). IL-2, IL-15, and IL-7 can all support NK cell differentiation, nevertheless, analyses of IL-2(-/-), IL-2Rα(-/-) mice fail to exhibit significant defects in NK cell development. This data suggest that IL-2 might have a redundant role in NK cell differentiation (4).

Molecular Weight: The 134 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 15,396 Da. The DTT-reduced protein migrates at approximately 14kDa and the non-reduced protein migrates with slightly greater mobility by SDS-PAGE.

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation