FIL1h Protein (AA 31-183)

Catalog No. ABIN2666729

This Recombinant FIL1h protein is expressed in Escherichia coli (E. coli).

Quick Overview for FIL1h Protein (AA 31-183) (ABIN2666729)

Target: FIL1h (Interleukin 1 eta (FIL1h))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 31-183

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: The ED50 is 0.1 - 0.5 ng/ml, corresponding to a specific activity 0.2 - 1.0 x 107 units/mg, as determined by a dose-dependent stimulation of IL-6 secretion in 3T3L1 preadipocytes.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA.

Buffer: 0.22 μm filtered protein solution is in 20 mM Tris pH 7.0, 150 mM, 2 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for one week, at -20°C for six months, or at -70°C for one year.

Target Details

Target: FIL1h (Interleukin 1 eta (FIL1h))

Alternative Name: IL-36 beta

Background: The IL-1 family is a group of cytokines comprised of 11 members, including the IL-36 cytokines, IL-36α, β and γ (previously known as IL-1F6, IL-1F8, and IL-1F9). Like other IL-36 cytokines, IL-36β signals through IL-1Rrp2 and IL-1RAcP and activate NF-κB and MAPKs. IL-36β functions as a chemokine and induces migration of bone marrow-derived dendritic cells (BMDCs) and CD4+ T cells. IL-36β induces proinflammatory cytokines, including IL-12, IL-1β, IL-6, and TNF-α in BMDCs. Furthermore, it has been shown that IL-36β synergizes with IL-12 to enhance Th1 polarization. IL-36β is also expressed in neurons and glia cells, but recombinant IL-36β does not induce the classical IL-1 response in brain cells. In a cultured keratinocyte system, IL-36β can induce robust expression of many chemokines in macrophages, T cells, and neutrophils. The secretion of antimicrobial peptides, including HBD-2, HBD-3, lipocalin 2, CAMP, elafin, serpinB1, and IL-8 can be induced by IL-36β in cultured keratinocytes. IL-36β, can also upregulate MMP9 and MMP19 mRNA in cultured keratinocytes. IL-36β has been implicated in the development of human psoriasis. IL-36β is expressed in the skin and the expression can be upregulated by IL-1β, TNF-α, flagellin, and poly (I:C). The expression of IL-36β is also increased in plaque psoriasis. In addition, IL-36β can be detected in mononuclear cells that infiltrate lesional skin. In mouse models of psoriasis, IL-36β is significantly upregulated in inflamed skin.

Molecular Weight: The 153 amino acid recombinant protein has a predicted molecular mass of approximately 17 kDa. The DTT-reduced and non-reduced protein migrates at approximately 17 kDa by SDS-PAGE. The predicted N-terminal amino acid is Ser.