FLT3LG Protein (AA 27-188)

Catalog No. ABIN2666813

This Recombinant FLT3LG protein is expressed in CHO Cells.

Quick Overview for FLT3LG Protein (AA 27-188) (ABIN2666813)

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: CHO Cells

Application: Flow Cytometry (FACS)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 27-188

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: ED50 = 5.0 - 25.0 ng/ml, corresponding to a specific activity of 0.4 - 2.0 x 105 units/mg, as determined by induction of IL-6 production on M1 cell.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in PBS, pH 6.0.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Target Details

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Alternative Name: FLT3L

Background: Mouse FLT3L was initially cloned from a murine T cell line, P7B-0.3A4, the human and mouse FLT3L proteins share 72 % amino acid identity. FLT3L is synthesized as a type I membrane-bound protein, which is cleaved to become a soluble growth factor. Additionally, a soluble form of FLT3L has been reported as a result of alternative splicing. TACE (ADAM17) plays a key role in the ectodomain shedding of FLT3L, in fact, serum FLT3L levels are decreased in TACE deficient mice. FLT3L is crucial for the development of the two main subsets of dendritic cells (DCs): conventional DCs (cDCs) and plasmacytoid DCs (pDCs). Changes in development or the number of DCs can alter T cell immunity and tolerance. A feedback loop between DCs and Tregs is regulated via FLT3L, as it has been shown that the increase in Tregs induced by DC expansion delays the onset of type 1 autoimmune diabetes and IBD in mice. Also, FLT3L facilitates formation of Tregs and thus, reduces severity of antigen-induced arthritis in mice. FLT3L is elevated in the synovial fluid of rheumatoid arthritis (RA) patients and FLT3L has been included in panels of preclinical markers for predicting the possible development of RA. The innate sensing pathway triggered by Plasmodium infection regulates DC homeostasis and adaptive immunity via FLT3L release. High levels of FLT3L and increased DCs have been detected in humans and mice during Plasmodium infection.

Molecular Weight: The 162 amino acid recombinant protein has a predicted molecular mass of approximately 18.3 kDa. The DTT-reduced and non-reduced protein migrate at approximately 20-26 kDa and 24-30 kDa by SDS-PAGE. The N-terminal amino acid is Gly.

Pathways: RTK Signaling