FLT3LG Protein (AA 27-185) (His tag)

Catalog No. ABIN7274656

Product Details

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Protein Type: Recombinant

Protein Characteristics: AA 27-185

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This FLT3LG protein is labelled with His tag.

Purpose: Human FLT3 Ligand Protein

Sequence: Thr27-Pro185

Characteristics: Recombinant Human FLT3 Ligand Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr27-Pro185.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human FLT3 Ligand, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 30ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Alternative Name: Flt3 Ligand (FLT3LG Products)

Synonyms: Flt3lg Protein, Ly72L Protein, FL Protein, FLT3L Protein, Vegfr3 Protein, fms related tyrosine kinase 3 ligand Protein, FMS-like tyrosine kinase 3 ligand Protein, fms-related tyrosine kinase 4 Protein, fms-related tyrosine kinase 3 ligand Protein, FLT3LG Protein, Flt3l Protein, Flt4 Protein, Flt3lg Protein

Background: Flt-3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.

Molecular Weight: 19.1 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Tris-Bis PAGE result.

Pathways: RTK Signaling