CD33 Protein (CD33) (AA 18-259, C-Term) (Fc Tag)

Catalog No. ABIN2666954

This Recombinant CD33 protein is expressed in HEK-293 Cells.

Quick Overview for CD33 Protein (CD33) (AA 18-259, C-Term) (Fc Tag) (ABIN2666954)

Target: CD33

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 18-259, C-Term

Purification tag / Conjugate: This CD33 protein is labelled with Fc Tag.

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.1 EU per μg of protein as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Recombinant human Siglec 3 is able to agglutinate human red blood cells with an ED50 = 0.5 - 4.0 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 50 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 0.2 - 1 % BSA or HSA. After dilution, the cytokine can be stored at 4 °C for one month or from -20 °C to -70 °C for up to three months.

Concentration: 100 μg/mL

Buffer: 0.22 μm filtered protein solution is in PBS, pH 7.2.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Target Details

Target: CD33

Alternative Name: Siglec 3

Background: Siglec proteins (sialic acid binding Ig-like lectins) are type I membrane proteins with an extracellular region containing a sialic acid binding V-set Ig-like domain at the N-terminus, followed by varying numbers of C2-set Ig domains. In the cytosolic domain, most Siglecs contain a combination of tyrosine motifs, including immunoreceptor tyrosine-based inhibitory motifs (ITIM), along with ITIM-like, Grb2-binding, and Fyn kinase sites. Siglecs are widely expressed on hematopoietic cells, often in a cell-type-specific manner. Their ligands, sialic acids, are negatively charged monosaccharides found on cell-surface glycoproteins and glycolipids. Studies suggest that Siglecs may participate in cell-cell interactions and act as receptors for the entry of viral or bacterial pathogens. In addition, the presence of ITIM indicates that these molecules play a role in the suppression of immunoreceptor signaling. Siglecs can be classified into two subgroups with Siglec 1, 2, and 4, as one group and a Siglec 3/CD33-related subgroup (Siglec 3 and 5 through 14) as the second. Siglec 3, also known as CD33 and GP67, is mainly expressed on myeloid cells and also on activated NK and T cells. Human Siglec 3 has two tyrosine residues in its cytoplasmic domain. When phosphorylated, these tyrosines could confer an inhibitory function. CD33 polymorphism has been associated with late-onset Alzheimer's disease.

Molecular Weight: This 493 amino acid recombinant protein has a predicted molecular mass of approximately 54.9 kDa. The protein migrates approximately at 70 kDa in DTT-reducing conditions and at 150 kDa in non-reducing conditions by SDS-PAGE. The predicted N-terminal amino