CXCL5 Protein (AA 37-114)

Catalog No. ABIN2666977

This Recombinant CXCL5 protein is produced in Escherichia coli (E. coli).

Quick Overview for CXCL5 Protein (AA 37-114) (ABIN2666977)

Target: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purity: > 98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 37-114

Endotoxin Level:

Less than 0.01 ng per μg cytokine as determine by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.,Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Prior to use, reconstitute the lyophilized powder with 0.2 mLof PBS containing a carrier protein (e.g., 1 % BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70 °C for up to one month.

Buffer: Lyophilized

Handling Advice: Use a manual defrost freezer and avoid repeated freeze thaw cycles.

Storage: 4 °C

Storage Comment: Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt.

Expiry Date: 12 months

Target Details

Target: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Alternative Name: CXCL5

Background: CXCL5 is a member of the CXC family of chemokines, also known as epithelial activated peptide 78 (ENA-78). The precursor of CXCL5 contains 114 amino acids, with a signal peptide of 36 amino acids which is cleaved to generate the 78 amino acid mature protein (8 kD). CXCL5 is expressed by monocytes/macrophages, endothelial cells, vascular smooth muscle cells, epithilial cells, and eosinophils. CXCL5 is an inflammatory cytokine which binds to the cell surface receptor CXCR2 and induces chemotaxis of neutrophils to a target site. High levels of CXCL5 have been associated with prostate, gastric, and pancreatic cancer progression.

Molecular Weight: The 78 amino acid recombinant protein has a predicted molecular mass of approximately 8.35 kDa. The DTT-reduced and non-reduced protein migrate at approximately 10 kDa by SDS-PAGE. The N-terminal amino acid is Ala.

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity