FGF10 Protein (AA 40-208, N-Term)

Catalog No. ABIN2667400

This Recombinant FGF10 protein is produced in Escherichia coli (E. coli).

Quick Overview for FGF10 Protein (AA 40-208, N-Term) (ABIN2667400)

Target: FGF10 (Fibroblast Growth Factor 10 (FGF10))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Purity: >95 % , as determined by Coomassie stained SDS-PAGE gel and HLPC analysis.

Product Details

Protein Characteristics: AA 40-208, N-Term

Endotoxin Level:

Less than 0.1 ng per μg of protein.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: ED50 ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2.0 x 106 units/mg, as measured by its ability to stimulate thymidine uptake by BaF3 cells expressing FGF receptors in a dose dependent manner.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in 5 mM sodium phosphate, pH 8.0 to a concentration of <0.5 mg/mL. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized, carrier-free.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored at -20°C or -70°C.

Target Details

Target: FGF10 (Fibroblast Growth Factor 10 (FGF10))

Alternative Name: FGF-10

Background: FGF-10, also named keratinocyte growth factor 2 (KGF-2), is a member of the fibroblast growth factor (FGF) family and is functionally similar to the other FGF family member FGF-7. FGF-10 acts as a paracrine growth factor and plays an important role in embryonic development, cell growth, and cell differentiation. During embryonic stages, FGF-10 is essential for lung development, promoting lung morphogenesis, branching, and airway extension. FGF-10 was revealed to participate in wound healing, promoting growth of epithelial cells in normal and wounded tissue. Increased expression of FGF-10 and the receptor FGFR2-IIIb has been reported to associate with various malignant diseases, such as prostate, breast, pancreas, and colorectum cancer, suggesting the role of FGF-10 in carcinogenesis.

Molecular Weight: The 170 amino acid recombinant protein has a predicted molecular mass of approximately 19.3 kDa. The predicted N-terminal amino acid is Met.

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Tube Formation, Positive Regulation of Response to DNA Damage Stimulus