FLT3LG Protein (AA 27-181)

Catalog No. ABIN2667412

This Recombinant FLT3LG protein is produced in Escherichia coli (E. coli).

Quick Overview for FLT3LG Protein (AA 27-181) (ABIN2667412)

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Purity: >98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 27-181

Endotoxin Level:

Less than 0.1 ng per μg of protein.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: ED50 ≤1.0 ng/ml, corresponding to a specific activity of ≥ 1.0 x 106 units/mg, as measured by its ability to stimulate the proliferation of human AML5 cells in a dose dependent manner.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized, carrier-free.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored at -20°C or -70°C.

Target Details

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Alternative Name: Flt-3 Ligand

Background: Fms-like tyrosine kinase 3 ligand is a hematopoietic cytokine structurally similar to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). Flt-3 ligand synergizes with other growth factors, such as interleukin-7 (IL-7) and CSFs, to promote proliferation and differentiation of early hematopoietic cells. In addition, Flt-3 ligand is involved in the commitment and expansion of dendritic cells as well as stimulating B cells, T cells, and NK cells. Both soluble form and membrane-bound forms of Flt-3 ligand are able to stimulate the cells expressing receptor tyrosine kinases Flt-3, transducing signals through the ERK and PI3K pathway. Flt-3 ligand is the only known ligand for Flt-3. Flt-3 and Flt-3 ligand have been implicated to participate in leukemogenesis, since the constitutively activated mutants of Flt-3 were found to be associated with the acute myeloid leukemia (AML).

Molecular Weight: The 155 amino acid recombinant protein has a predicted molecular mass of approximately 17.6 kDa. The predicted N-terminal amino acid is Thr.

Pathways: RTK Signaling