IL-10 Protein (AA 19-178)

Catalog No. ABIN2667449

This Recombinant IL-10 protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL-10 Protein (AA 19-178) (ABIN2667449)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Biochemical Assay (BCA)

Purity: Purity is >98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 19-178

Sterility: 0.22 μm filtered

Endotoxin Level:

Endotoxin level is <0.1 EU/μg (<0.01ng/μg) protein as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: ED50 = 1- 2 ng/ml, corresponding to a specific activity of 1- 0.5 x 106 units/mg, as determined by the dose dependent stimulation MC/9 cell proliferation.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in 20 mM Tris pH 7.5.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Target Details

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL-10

Background: IL-10 was first described as a cytokine that is produced by T helper 2 (Th2) cell clones. It inhibits interferon (IFN)-g synthesis in Th1 cell, and therefore it was initially called cytokine synthesis inhibiting factor (CSIF). Macrophages are the main source of IL-10 and its secretion can be stimulated by endotoxin (via Toll-like receptor 4, NF-κB dependent), tumor necrosis factor TNF-α (via TNF receptor p55, NF-kB-dependent), catecholamines, and IL-1. IL-10 controls inflammatory processes by suppressing the expression of proinflammatory cytokines, chemokines, adhesion molecules, as well as antigen-presenting and costimulatory molecules in monocytes/macrophages, neutrophils, and T cells. IL-10 inhibits the production of proinflammatory mediators by monocytes and macrophages such as endotoxin- and IFN-g-induced release of IL-1a, IL-6, IL-8, G-CSF, GM-CSF, and TNF-a. In addition, it enhances the production of anti-inflammatory mediators such as IL-1RA and soluble TNFα receptors. IL-10 inhibits the capacity of monocytes and macrophages to present antigen to T cells. This is realized by down-regulation of constitutive and IFNγ-induced cell surface levels of MHC class II, of costimulatory molecules such as CD86 and of some adhesion molecules such as CD58.

Molecular Weight: The 161 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 18.9 kDaa. The DTT-reduced protein migrates at approximately 18kDa and non-reduced protein migrates at approximately 15kDa by SDS-PAGE.

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints