IL-17A/F Protein (Heterodimer)

Catalog No. ABIN2667474

Recombinant IL-17A/F protein expressed in Escherichia coli (E. coli).

Quick Overview for IL-17A/F Protein (Heterodimer) (ABIN2667474)

Target: IL-17A/F (Interleukin-17 A/F Heterodimer (IL-17A/F))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS)

Purity: Purity is >98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: Heterodimer

Sterility: 0.22 μm filtered

Endotoxin Level:

Endotoxin level is < 0.1 EU/μg (< 0.01 ng/μg) protein as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Activity was tested by induction of IL-6 in human skin fibroblasts by IL-17A/F. The ED50 is 15-25 ng/ml, corresponding to a specific activity of 6.6 - 4 x 104 units/mg.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in 10 mM NaH2PO4, 300 mM NaCl, pH 7.2.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Target Details

Target: IL-17A/F (Interleukin-17 A/F Heterodimer (IL-17A/F))

Alternative Name: IL-17A/F

Background: IL-17A/F is part of the IL-17 cytokine family which consists of six structurally related proteins (IL-17A, B, C, D, E, and F). IL-17A is expressed primarily by Th17 cells, a subset of CD4 T cells. IL-17F is most closely related to IL-17A. The two molecules share 50 % aminoacid sequence homology. Like IL-17A, IL-17F mRNA and protein have been detected in Th17 cells. IL-17F and IL-17A exist as a homodimers, adopting a cysteine knot motif formed through the interactions of four cysteines, one of which is responsible for the interchain bonding. IL-17F and IL-17A can form both homodimeric and heterodimeric proteins when expressed in a recombinant system and all forms of the recombinant proteins have in vitro functional activity. In addition, activated human CD4-T cells produce the homodimers of IL-17F, IL-17A, and the IL-17F/IL-17A heterodimer.

Molecular Weight: Recombinant hIL-17A/F is a disulfide linked heterodimer consisting of N-terminal methionylated hIL-17A and non-methionylated hIL-17F. The recombinant heterodimer has a predicted molecular mass of 30,552 Da. The DTT-reduced protein migrates as monomers, at