IL-17A/F Protein (AA 26-158) (His-Avi Tag)

Catalog No. ABIN7274968

Product Details

Target: IL-17A/F (Interleukin-17 A/F Heterodimer (IL-17A/F))

Protein Type: Recombinant

Protein Characteristics: AA 26-158

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This IL-17A/F protein is labelled with His-Avi Tag.

Purpose: Mouse IL-17A & F Protein

Sequence: Ala26-Ala158 (IL-17A) & Arg29-Ala161 (IL-17F)

Specificity: Uni-Prot: Q62386 (IL-17A), Q7TNI7-1 (IL-17F)

Characteristics: Recombinant Mouse IL-17A & F Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala26-Ala158(IL-17A) & Arg29-Ala161(IL-17F).

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Mouse IL-17A&F, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse IL-17R alpha, hFc Tag with the EC50 of 19.8ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-17A/F (Interleukin-17 A/F Heterodimer (IL-17A/F))

Alternative Name: IL-17A&F (IL-17A/F Products)

Synonyms: il17-5 Protein, si:ch211-63o20.2 Protein, interleukin 17a/f3 Protein, il17a/f3 Protein

Background: Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.

Molecular Weight: 17.9 kDa(IL-17A)&14.9 kDa(IL-17F). Due to glycosylation, the protein migrates to 22-25 kDa&27-30 kDa based on Tris-Bis PAGE result.

UniProt: Q62386