IL17F Protein (AA 29-161)

Catalog No. ABIN2667481

This Recombinant IL17F protein is produced in Escherichia coli (E. coli).

Quick Overview for IL17F Protein (AA 29-161) (ABIN2667481)

Target: IL17F (Interleukin 17F (IL17F))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Multiplex Assay (MA)

Purity: >98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 29-161

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: ED50 =300 to 600 ng/ml, corresponding to a specific activity of 0.16 - 0.33 x 104 units/mg, as determined by a dose dependent stimulation of fetal mouse skin fibroblasts production of CXCL1.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in 10 mM NaH2PO4, 0.15 M NaCl, pH 7.2.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Target Details

Target: IL17F (Interleukin 17F (IL17F))

Alternative Name: IL-17F

Background: IL-17F belongs to the IL-17 cytokine family which includes IL-17A, B, C, D, and E (also called IL-25). IL-17F shares the strongest homology to IL-17A. They share 50 % amino acid sequence homology. Recently, an IL-17-IL-17F heterodimer was found to be expressed in Th17 cells together with IL-17 and IL-17F homodimers. IL-17 and IL-17F gene promoters share the same pattern of chromatin remodeling in differentiated Th17 cells. T cell populations in vitro and in vivo have shown different ratios of IL-17 and IL-17F expression. IL-17, IL-17F regulates proinflammatory gene expression in vitro. Studies in vivo using Il17a(-/-), Il17f(-/-), and Il17a(-/-)Il17f(-/-) mice showed that IL-17F played only marginal roles, if at all, in the development of delayed-type and contact hypersensitivities, autoimmune encephalomyelitis, collagen-induced arthritis, and arthritis in Il1rn(-/-) mice. In contrast, both IL-17F and IL-17A were involved in host defense against mucoepithelial infection by Staphylococcus aureus and Citrobacter rodentium. Additional studies in vivo have suggested a role for IL-17F in the induction of neutrophilia in the lungs and in the exacerbation of Ag-induced pulmonary inflammation.

Molecular Weight: The 134 amino acid recombinant protein has a predicted molecular mass of 15014 Da and contains Methionine as N-terminal. This protein exists as a disulfide-linked homodimer. The DTT-reduced protein migrates at approximately 15 kDa by SDS-PAGE. The non-red

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity