IL17F Protein (AA 31-163) (His tag)

Catalog No. ABIN7275000

This Recombinant IL17F protein is produced in HEK-293 Cells.

Quick Overview for IL17F Protein (AA 31-163) (His tag) (ABIN7275000)

Target: IL17F (Interleukin 17F (IL17F))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 31-163

Purification tag / Conjugate: This IL17F protein is labelled with His tag.

Purpose: Cynomolgus IL-17F Protein

Sequence: Arg31-Gln163

Characteristics: Recombinant Cynomolgus IL-17F Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg31-Gln163.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human IL-17R alpha, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus IL-17F, His Tag with an affinity constant of 0.13 uM as determined in SPR assay (Biacore T200).,Cynomolgus IL-17F on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus IL-17F is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL17F (Interleukin 17F (IL17F))

Alternative Name: IL-17F

Background: The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17A through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus.IL-17F is ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover.

Molecular Weight: 16.1 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Bis-Tris PAGE result.

UniProt: G7P4V0

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity