VEGFA Protein (AA 27-146)

Catalog No. ABIN2667604

This Recombinant VEGFA protein is produced in Escherichia coli (E. coli).

Quick Overview for VEGFA Protein (AA 27-146) (ABIN2667604)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Purity: Purity is >98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 27-146

Sterility: 0.22 μm filtered

Endotoxin Level:

Endotoxin level is <0.1 EU/μg (<0.01 ng/μg) protein as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: ED50 = 1-4 ng/ml, corresponding to a specific activity of 1-0.25 x 106 units/mg, as determined by the dose dependent stimulation of HUVEC cells proliferation.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22μm filtered protein solution is in 5 mM Citric Acid, 5 mM NaH2PO4, 150 mM NaCl, pH 4.0.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Target Details

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGF120

Background: VegfA belong to the VEGF family which has at least 7 members, VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, PlGF and snake venom-derived VEGFs such as T.f. (Trimeresurus flavoviridis) svVEGF. In humans, VEGF-A is highly expressed in most of the solid tumors generated in breast, lung, renal, colorectal and liver tissues. VEGF-A has strong vascular permeability activity, and significantly contributes to the formation of ascites tumors. VEGF can act as a direct proinflammatory mediator during the pathogenesis of RA, and protect rheumatoid synoviocytes from apoptosis, which contributes to synovial hyperplasia. VEGF is expressed in synovial macrophages and synovial fibroblasts in the synovial tissues of RA patients.

Molecular Weight: The 120 amino acid recombinant protein has a predicted molecular mass of approximately 14,071 Da. This protein exists as a disulfide-linked homodimer. The DTT-reduced protein migrates at approximately 15kDa and the non-reduced protein migrates as a homodi

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling