Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

VEGFA Protein (AA 27-190) (His tag,AVI tag,Biotin)

VEGFA Origin: Mouse Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
Catalog No. ABIN4949052
  • Target See all VEGFA Proteins
    VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 27-190
    Origin
    • 24
    • 19
    • 15
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Source
    • 41
    • 17
    • 10
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This VEGFA protein is labelled with His tag,AVI tag,Biotin.
    Application
    Functional Studies (Func)
    Brand
    MABSol®,PrecisionAvi
    Sequence
    AA 27-190
    Specificity
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    Characteristics
    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 22.5 kDa. As a result of different glycosylation, the protein migrates as 28-33 kDa under reducing (R) condition, and 46-60 kDa under non-reducing (NR) condition (SDS-PAGE).
    Purity
    >95 % as determined by SDS-PAGE.
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product VEGFA Protein
  • Comment

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Handling Advice
    Please avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
  • Target
    VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
    Alternative Name
    VEGF164 (VEGFA Products)
    Synonyms
    MVCD1 Protein, VEGF Protein, VPF Protein, Vegf Protein, Vegf120 Protein, Vegf164 Protein, Vegf188 Protein, Vpf Protein, vegf Protein, vegfa Protein, wu:fj82c06 Protein, VEGF-A Protein, VEGF164 Protein, eVEGF120 Protein, eVEGF164 Protein, vegf-a Protein, vpf Protein, vefg Protein, si:dkey-14d8.3 Protein, wu:fd42e02 Protein, vascular endothelial growth factor A Protein, vascular endothelial growth factor Aa Protein, vascular endothelial growth factor A L homeolog Protein, vascular endothelial growth factor Ab Protein, VEGFA Protein, Vegfa Protein, vegfaa Protein, vegfa.L Protein, vegfa Protein, vegfab Protein
    Background
    Vascular endothelial growth factor A (VEGFA) is also known as Vascular permeability factor (VPF). VEGFA belongs to the PDGF/VEGF growth factor family. VEGFA is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. VEGFA is produced by a group of three major isoforms as a result of alternative splicing and if any three isoforms are produced (VEGFA120, VEGFA164, and VEGFA188) then this will not result in vessel defects and death of the full VEGFA knockout in mice.
    Molecular Weight
    22.5 kDa
    Pathways
    RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
You are here:
Support