WISP1 Protein (AA 23-367)

Catalog No. ABIN2667610

This Recombinant WISP1 protein is produced in Escherichia coli (E. coli).

Quick Overview for WISP1 Protein (AA 23-367) (ABIN2667610)

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Purity: >98 % , as determined by SDS-PAGE gel and HPLC analysis.

Product Details

Protein Characteristics: AA 23-367

Endotoxin Level:

Less than 0.1 ng per μg of protein.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: The ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 1.0 - 3.0 μg/ml.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. Note: Slow to dissolve. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1 % BSA and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored at -20°C or -70°C.

Target Details

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Alternative Name: WISP-1

Background: WNT1 inducible signaling protein (WISP) is a protein subfamily that belongs to the connective tissue growth factor (CTGF) family. This family includes connective tissue growth factor (CTGF), cysteine-rich-61 (CYR61), and nephroblastoma overexpressed (NOV). CTGF is a family that is cysteine-rich, carries four conserved domains from N- to C-terminus: insulin-like growth factor binding protein (IGFBP) domain, von Willebrand type C repeats (vWC) domain, thrombospondin type 1 repeat (TSR) domain, and a C-terminal domain (CT) with a cysteine knot motif. CTGF family is comprised of several extracellular matrix associated proteins that contribute to cell adhesion, migration, and wound repair. CTGF family member WISP-1, like WISP-2, and WISP-3, is a secreted extracellular matrix associated signaling protein that plays an important role in vessel formation, and bone and cartilage development. WISP-1 stimulates mesenchymal cell proliferation and osteoblastic differentiation. It is a downstream regulator of Wnt/Frizzled signaling that mediates various developmental processes and thus was identified as an oncogene modulated by the Wnt-1-β -catenin pathway. It is also known that WISP-1 is involved in prevention of late stage p53-mediated cell death and elevates expression of antiapoptotic Bcl-xL. WISP-1 overexpression, on the other hand, is associated with tumor formation due to its critical roles in early stage cellular proliferation and survival through phosphatidylinositol-3-kinase (PI3-K) and protein kinase B (Akt) pathways.

Molecular Weight: The 345 amino acids recombinant protein has a predicted molecular mass of 38.1 kDa. The predicted N-terminal amino acid is Thr.

Pathways: WNT Signaling, Growth Factor Binding