Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

PRMT5 Protein (DYKDDDDK Tag)

PRMT5 Origin: Human Host: Baculovirus Recombinant EAA, ScA
Catalog No. ABIN2669658
  • Target See all PRMT5 Proteins
    PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))
    Protein Type
    Recombinant
    Origin
    • 7
    • 1
    Human
    Source
    • 3
    • 2
    • 2
    • 1
    • 1
    Baculovirus
    Purification tag / Conjugate
    This PRMT5 protein is labelled with DYKDDDDK Tag.
    Application
    Enzyme Activity Assay (EAA), Screening Assay (ScA)
    Characteristics
    Recombinant PRMT5 (accession number NP_006100.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 74.2 kDa.
    Top Product
    Discover our top product PRMT5 Protein
  • Application Notes
    Recombinant PRMT5 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3R8 and H4R3 methyltransferase. HMT Assay Conditions:50 mM TrisCl, pH 8.6, 0.02 % Triton X-100, 2 mM MgCl2, 1 mM TCEP, 60 ng/μl H3 (1-21) peptide, 20 ng/μl Recombinant PRMT5 protein at 2 hours at room temperature. Activity was detected by fluorography.
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
    Storage
    -80 °C
    Storage Comment
    Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
  • Target
    PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))
    Alternative Name
    PRMT5 (PRMT5 Products)
    Synonyms
    HRMT1L5 Protein, IBP72 Protein, JBP1 Protein, SKB1 Protein, SKB1Hs Protein, Jbp1 Protein, Skb1 Protein, si:zc14a17.2 Protein, skb1 Protein, wu:fb95f10 Protein, zgc:110669 Protein, hsl7 Protein, DDBDRAFT_0187422 Protein, DDBDRAFT_0235403 Protein, DDB_0187422 Protein, DDB_0235403 Protein, NV18830 Protein, jbp1 Protein, ibp72 Protein, skb1hs Protein, hrmt1l5 Protein, protein arginine methyltransferase 5 Protein, protein arginine N-methyltransferase 5 Protein, protein arginine methyltransferase 5 L homeolog Protein, putative arginine N-methyltransferase, type II Protein, Skb1 family protein Protein, Protein arginine N-methyltransferase 5 Protein, PRMT5 Protein, Prmt5 Protein, prmt5 Protein, prmt5.L Protein, LOC100115766 Protein, prmt-5 Protein
    Background
    PRMT5 (Protein Arginine Methyltransferase 5, also known as JBP1, Janus Binding Protein 1) is a type II arginine methyltransferase, a protein that transfers methyl groups to the arginine residues of substrate proteins. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT5 symmetrically dimethylates histone H2A Arg3, H3 Arg8 and H4 Arg3. It also methylates Piwi proteins which regulate small non-coding RNAs and snRNPs, regulating snRNP core particle assembly. PRMT5 functions as a co-repressor of transcription and is recruited by a number of DNA binding proteins and transcriptional repressor complexes. It is present both in the nucleus and the cytoplasm. It also interacts with COPR5, JAK2, PRDM1 and RPS10.
    Molecular Weight
    74.2 kDa
    Pathways
    Chromatin Binding, Regulation of Muscle Cell Differentiation, Ribonucleoprotein Complex Subunit Organization, Skeletal Muscle Fiber Development
You are here:
Support