LSD1 Protein (DYKDDDDK Tag,His tag)

Catalog No. ABIN7939236

This Recombinant LSD1 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for LSD1 Protein (DYKDDDDK Tag,His tag) (ABIN7939236)

Target: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Purity: >91 % pure by SDS-PAGE

Product Details

Purification tag / Conjugate: This LSD1 protein is labelled with DYKDDDDK Tag,His tag.

Purpose: Recombinant LSD1 / KDM1A protein

Characteristics: Recombinant LSD1 / KDM1A (accession number NP_055828) was expressed in Sf9 cells and contains an N-terminal FLAG tag with a molecular weight of 97.1 kDa. LSD1 (Lysine-specific demethylase 1, also designated KDM1A) was identified as a subunit of different complexes (such as CTBP, CoREST, NuRD or BRAF35). LSD1 is involved in transcriptional repression of genes through the demethylation of Histone H3 monomethyl Lys4 and Histone H3 dimethyl Lys4, a methylation site associated with transcriptional activation. LSD1 is also involved in transcriptional activation through the demethylation of Histone H3 monomethyl Lys9 and Histone H3 dimethyl Lys9, a modification often associated with transcriptional repression. LSD1 is also able to demethylate Lys370me1 and Lys370me2 in the regulatory domain of the tumor suppressor p53. LSD1 is a flavin-dependent amine oxidase. Recombinant LSD1 / KDM1A can be used to study enzyme kinetics and perform inhibitor screening.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Full length recombinant KDM1A / LSD1 protein was expressed in Sf9 cells and is supplied in 25 mM Hepes pH 7.5, 300 mM NaCl, 20 % Glycerol, 0.04 % Triton X-100, and 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Alternative Name: LSD1 / KDM1A

Background:

Protein Background: LSD1 (Lysine-specific demethylase 1, also designated KDM1A) was identified as a subunit of different complexes (such as CTBP, CoREST, NuRD or BRAF35). LSD1 is involved in transcriptional repression of genes through the demethylation of Histone H3K4me1 and Histone H3K4me2, a methylation site associated with transcriptional activation. LSD1 is also involved in transcriptional activation through the demethylation of Histone H3K9me1 and Histone H3K9me2, a modification often associated with transcriptional repression. LSD1 is also able to demethylate Lys370me1 and Lys370me2 in the regulatory domain of the tumor suppressor p53. LSD1 is a flavin-dependent amine oxidase.

Short Description: Recombinant LSD1 / KDM1A (accession number NP_055828) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with an observed molecular weight of 110 kDa. The recombinant protein is >91 % pure by SDS-PAGE.

Aliases: Lysine Demethylase 1A, Flavin-Containing Amine Oxidase Domain-Containing Protein 2, Lysine-Specific Histone Demethylase 1

Molecular Weight: 97.1 kDa

NCBI Accession: NP_055828

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling, Warburg Effect